The Invertebrate Hall exhibition is arranged systematically, starting with the most primitive animals: sponges, followed by cnidarians, worms, molluscs, echinoderms, and arthropods.

The hall showcases over 4,500 invertebrate specimens from Lithuania and other parts of the world. Some of these were collected by the museum’s founder, Professor T. Ivanauskas. A significant portion of the exhibits includes tropical marine animals donated by Professor Pranciškus Baltrus Šivickis, as well as marine shells gifted by Australian Lithuanian Elena Liutikienė between 1973 and 1976.

In 2008, former seaman Vykintas Matuzevičius donated a collection of over 10,000 shells, which he had gathered over approximately 40 years. This collection features more than 2,000 species of marine and terrestrial snails and bivalve shells from various global locations. Since 2016, a portion of this donated collection has been on display in the museum hall.

The Invertebrate Hall also exhibits the largest bivalve mollusc found in Lithuania, the great pond mussel, discovered in 1999.

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